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KMID : 0350519930460010247
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Journal of Catholic Medical College
1993 Volume.46 No. 1 p.247 ~ p.254
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Effect of in Vitro T cell Depletion in Mice Allogeneic Marrow Graft to Survival and Immunological Reconstitution
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Abstract
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Allogeneic bone marrow transplantation can provide curative therapy for patients with several refractory disorders such as hematopoietic malignant disorders, severe aplastic anemia, severe combined immunodeficiency, and some genetic disorders.
However its widespread use has been limited by the high incidence of graft-versus-host disease(GVHD). Mediators of GVHD are alloreactive donor T lymphocytes that are cytotoxic to host cells.
In present study, preparing the chimera made by infusing allogeneic marrow cells treated with anti-Thy 1.2 antibody in vitro to remove donor T cells after 1,000 cGy total body iffadiation, we compared the survival of the chimera with the recipient mice
which had received untreated donor marrow inoculum containing T cells and also evaluated immunological reconstitution of the chimeras by means of mixed lymphocyte culture and mitogen stimulation test on 100 days after marrow engraftment.
@ES The results were as follows :
@EN 1. Survival of the chimera treated to remove T cells was significantly improved than that of the recipinet mice received untreated marrow cells on 100 days after marrow engraftment(70% vs. 45%).
2. Chimeric spleen cells were found to be almost(95%) of donor origin in H-2 phenotype.
3. The spleen cells of the chimeras showed no proliferative response against donor or host cells in mixed lymphocyte culture.
4. Proliferative responses of chimeric spleen cells to optimal concentration of three mitogens were significantly increased irrespective of removal of T cells in inoculum.
These results support that T cell depletion from graft in allogeneic bone marrow transplantation inhibited GVHD and improved survival by suppression of GVHD in mice. And the reconstituted chimeras showed good immunologic reactivity without any
suppression.
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